Target Identification & Validation
Novel targets and science
- Data mining / bioinformatics
- Interaction of target with ligand, e.g. binding validation
- Analysis of molecular signaling pathways
Services
- in-vitro and in-vivo models (see Preclinical Development)
- Antisense technology
- Transgenics, incl. CRISPR/Cas and Sleeping Beauty Transposon System
- RNAi, e.g. in pain research
- RNAi and CRISPR-Cas screening
- Reporter und enzyme assays
Disease association
- patient samples and human cohorts (e.g. NAKO, pregnancy, renal and cardio diseases, preeclampsia)
- Tumor models with data about histology, genetic profile and drug sensitivity
- patient-derived 3D cell models
Analytics
- DNA, peptide and protein microarrays
- Genetic profiling (e.g. RT-PCR, Illumina, NGS)
- Protein profiling (e.g. DigiWest, Luminex, ProteinSimple, ELISA, LC-MS, Maldi-Tof, HPLC/LC/MS)
- Flow cytometer (quant.)
- Target-ligand binding analysis, e.g. surface plasmon resonance, NMR or using biophysical analysis
- Electrophysiology (manual and automated patch clamp, ion channel drug discovery, oocyte recording, Ca2+ imaging, CiPA assays, MEA-based assays)